4-(2-羟乙基)-1-哌嗪丙磺酸

性状描述: 白色晶体粉末。
熔点：237-239℃
pH range 7.3 - 8.7
pKa (25 °C) 8.0
pKa  8

质量标准: 项目                                 生化试剂级
                                        BC
纯度assay                             ≥99%(T)
水分Moistrue                          ≤0.5%
pH(1% in H2O)                         5.0-6.5



品级Grade                               超纯级  
                                    (Ultra pure,≥99.5% (T))
外观Appearance                         白色粉末
含量assay                              ≥99.5% (titration)
磷Phosphorus (P)                       ≤0.005%
不溶物Insoluble matter                 ≤0.1%
灼烧残渣Residue on ignition            ≤0.1%
pH                                     5.0-7.0 (20 °C, 1 M in H2O)
useful pH range                        7.3 - 8.7
pKa                                    8
水溶性solubility H2O:                  soluble1 M at 20 °C, clear, colorless
溴bromide (Br-):                       ≤0.1%
  Al:                                  ≤0.0005%
  Ca:                                  ≤0.002%
  Cu:                                  ≤0.0005%
  Fe:                                  ≤0.0005%
  K:                                   ≤0.005%
  Mg:                                  ≤0.0005%
  NH4+:                                 ≤0.1%
  Na:                                  ≤0.005%
  Pb:                                  ≤0.001%
  Zn:                                  ≤0.0005%
A₂₆₀(1M,water):                       ≤0.1
A₂₈₀(1M,water):                       ≤0.1



品级Grade                               细胞培养级  
                                  (for cell culture, ≥99.5%)
外观Appearance                         白色粉末
含量assay                              ≥99.5% (titration)
heavy metals (as Pb):                  ≤5 ppm
灼烧残渣Residue on ignition            ≤0.1%  
水溶性solubility H2O:                  soluble1 M at 20 °C, clear, colorless
A₂₆₀(1M,water):                       ≤0.1
A₂₈₀(1M,water):                       ≤0.1
DNA酶DNases,                           none detected
RND酶RNases,                           none detected
磷酸酶phosphatases,                    none detected
蛋白酶proteases,                       none detected
内切酶Endonuclease,                    none detected
内毒素Endotoxin                        ≤ 0.1 EU/MG
标准平板计数Standard plate count       ≤100 CFU/G
细胞培养测试cell culture test          pass

储存条件: 密封避光保存。

用途: HEPPS有很多相似于HEPES的特性。因为有高的缓冲范围，适合用于磷酸化反应，特别是Tricine不能使用的情况下。HEPPS可用于Folin蛋白检测中，但不能用于缩二脲检测